Evaluation of eleven reference genes for Reverse Transcriptase Quantitative PCR of rubber tree under water deficit Evaluasi sebelas gen referensi untuk Reverse Transcriptase Quantitative PCR pada tanaman karet tercekam kekeringan
نویسندگان
چکیده
منابع مشابه
PCR - Based Reverse Transcriptase Assay
ES cells. This phenomenon has been observed in our laboratory in a number of cases. Clones putatively identified as homologous recombinants by Southern analysis can be quickly checked for random insertion of extra copies of the targeting vector by PCR. Given the small difference in size between the two alleles and use of only two primers common to both WT and mutant DNA, the amplification rate ...
متن کاملReverse transcriptase-polymerase chain reaction (rt-PCR).
Reverse transcriptase polymerase chain reaction (RT-PCR) has become a well-established and powerful molecular technique for studying ribonucleic acids. It is used in medical diagnostics for detecting viral RNA, in hematology and oncology for detecting chimeric transcripts of rearranged genes (1), and in the broad area of research applications in gene expression studies. Since its introduction (...
متن کاملA sensitive and quantitative single-tube real-time reverse transcriptase-PCR for detection of enteroviral RNA.
BACKGROUND Enteroviruses (EVs) are significant human pathogens. Rapid and sensitive diagnostic techniques are desirable. OBJECTIVES To develop a quantitative single-tube real-time reverse transcription-polymerase chain reaction (RT-PCR) for human enterovirus ribonucleic acid (RNA) (QPCR), with protection against amplimer contamination. STUDY DESIGN The method was evaluated with serial dilut...
متن کاملReverse transcriptase (RT) inhibition of PCR at low concentrations of template and its implications for quantitative RT-PCR.
Numerous instances of reverse transcriptase (RT) inhibition of the PCR were observed while developing nonquantitative uncoupled RT-PCR techniques for detecting nitrogenase and ammonia monooxygenase gene expression in situ. The inhibitory effect of RT on the PCR was removed with increasing template concentrations beyond 10(5) to 10(6) copies. Including T4 gene 32 protein during the reverse trans...
متن کاملQuantification of murine cytokine mRNAs using real time quantitative reverse transcriptase PCR.
Recently, a novel technique for "real time" quantitative Reverse Transcriptase-PCR which measures PCR-product accumulation during the exponential phase of the PCR reaction using a dual-labelled fluorogenic probe, has been developed. This method allows direct detection of PCR-product formation by measuring the increase in fluorescent emission continuously during the PCR reaction. Here we present...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: E-Journal Menara Perkebunan
سال: 2016
ISSN: 1858-3768,0125-9318
DOI: 10.22302/ppbbi.jur.mp.v83i2.5